Antileishmanial and Antiplasmodial Activities of Secondary Metabolites from the Root of Antrocaryon klaineanum Pierre (Anacardiaceae)

Antrocaryon klaineanum is traditionally used for the treatment of back pain, malaria, female sterility, chlamydiae infections, liver diseases, wounds, and hemorrhoid. This work aimed at investigating the bioactive compounds with antileishmanial and antiplasmodial activities from A. klaineanum. An unreported glucocerebroside antroklaicerebroside (1) together with five known compounds (2–6) were isolated from the root barks of Antrocaryon klaineanum using chromatographic techniques. The NMR, MS, and IR spectroscopic data in association with previous literature were used for the characterization of all the isolated compounds. Compounds 1–4 are reported for the first time from A. klaineanum. The methanol crude extract (AK-MeOH), the n-hexane fraction (AK-Hex), the dichloromethane fraction (AK-DCM), the ethyl acetate fraction (AK-EtOAc), and compounds 1–6 were all evaluated for their antiparasitic effects against Plasmodium falciparum strains susceptible to chloroquine (3D7), resistant to chloroquine (Dd2), and promastigotes of Leishmania donovani (MHOM/SD/62/1S). The AK-Hex, AK-EtOAc, AK-MeOH, and compound 2 were strongly active against Dd2 strain with IC50 ranging from 2.78 ± 0.06 to 9.30 ± 0.29 µg/mL. Particularly, AK-MeOH was the most active—more than the reference drugs used—with an IC50 of 2.78 ± 0.06 µg/mL. The AK-EtOAc as well as all the tested compounds showed strong antileishmanial activities with IC50 ranging from 4.80 ± 0.13 to 9.14 ± 0.96 µg/mL.


Introduction
In the past decade, interest in traditional medicinal plants has increased exponentially with the arrival of new pathogens, resistance to existing drugs, climate change, and amidst all of this, the side effects of some synthetic drugs [1,2]. In Cameroon, malaria is present throughout the country and leishmaniasis in the northern regions; they are the two main diseases caused by tropical insects with the highest number of deaths in the last 10 years. Moreover, malaria represents the most widespread disease, with about 6.7 million cases and 13 839 deaths in 2021 [3]. Primarily, the number of people dying from malaria and leishmaniasis is due to the length of treatment, low efficacy, high cost, numerous side effects, induction of parasite resistance and, above all, vaccines that will not be available soon [1,4]. Thus, the research and promotion of local traditional medicinal plants as a source of new and natural medicines has become a high priority [2,[4][5][6]. Anacardiaceae is a family of plants known for their nutritional and therapeutic importance. Anacardiaceae plants are composed of trees or shrubs with about 81 genera grouped into 800 species including the genus Antrocaryon and the Antrocaryon klaineanum Pierre species [7]. In Cameroon, three main species of the genus Antrocaryon are well known: Antrocaryon guillaumin, A. micraster, and A. klaineanum. Antrocaryon klaineanum is called "White mahogany (English)", "Bouton d'antilope (French)" [8]. It is a tree about 35 m high that is well known by the population for the primary healthcare treatment for ailments such as back pain, malaria, female sterility, chlamydiae infections, liver diseases, wounds, etc. [7,8]. This work focused on the search for bioactive compounds and was motivated by previous studies conducted on A. klaineanum which mentioned the presence of a panel of bioactive secondary metabolites well known for their antiplasmodial activities. Previous phytochemical study of A. klaineanum have shown that flavonoids, ellargic acid, gallic acid, steroids, triterpenoids, and saponins are the main classes of secondary metabolite found in the genus Antrocaryon as well as in Antrocaryon klaineanum [7][8][9]. To the best of our knowledge, only the antiplasmodial activities of Antrocaryon klaineanum have already been reported [7,9]. This research work focuses on the chemical study and the antiplasmodial and antileishmania activities of the root barks of A. klaineanum. The chemical study led to the isolation and the characterization of six secondary metabolites, among which an unreported glucocerebroside derivative (1), together with five known compounds (2-6)-among which two coumarins (2)(3), an ellagic acid (4), and two steroids (5)(6). The compounds 1-4 are reported for the first time from A. klaineanum. The methanol crude extract (AK-MeOH), the n-hexane fraction (AK-Hex), the dichloromethane fraction (AK-DCM), the ethyl acetate fraction (AK-EtOAc) and compounds 1-6 were all evaluated for their antiparasitic effects against Plasmodium falciparum strains susceptible to chloroquine (3D7) and resistant to chloroquine (Dd2) and promastigotes of Leishmania donovani (MHOM/SD/62/1S).

Results
The methanol crude extract of the air-dried root barks of A. klaineanum was subjected to liquid-liquid extraction. The n-hexane, dichloromethane, and ethyl acetate fractions were all fractionated and purified using column chromatography (CC) over silica gels as well as using sephadex LH-20, which led to the isolation of six secondary metabolites-among which an unreported glucocerebroside, antroklaicerebroside (1), together with five known compounds (2)(3)(4)(5)(6) (Figure 1). The structures of all these compounds were determined based on the interpretation of NMR and MS analysis in comparison to reported data. known compounds (2)(3)(4)(5)(6) (Figure 1). The structures of all these compounds were determined based on the interpretation of NMR and MS analysis in comparison to reported data.   Figures S2-S4). The UV spectrum revealed maximum absorptions at 269 nm ( Figure S1). The IR spectrum ( Figure S6) of compound 1 revealed some characteristic absorption bands of hydroxyl (3 324 cm −1 ), amide (3 269 cm −1 ), trans-double bond (1 618 and 963 cm −1 ), and aliphatic chain (2 918 and 2 850 cm −1 ) [10][11][12]. The presence of these functional groups was in agreement with atoms found in the molecular formula and further supported by the 1D-and 2D-NMR.

Antiplasmodial, Antileishmanial, and Cytotoxicity Activities of Extract, Fractions, and Compounds from Antrocaryon Klaineanum
For this purpose, the crude extract, fractions, and isolated compounds from Antrocaryon klaineanum were assessed in vitro for their antileishmanial and antiplasmodial activities against L. donovani promastigotes 1S (MHOM/SD/62/1S) and both P. falciparum chloroquinesensitive (Pf 3D7) and chloroquine-resistant (Pf Dd2) strains ( Table 2, Figures 4 and 5). They were also assessed for cytotoxicity on Raw 264.7 macrophage cells ( Table 2). Based on the antiplasmodial activity classification criteria of Rasoanaivo et al. [29], the crude extract (AK-MeOH) strongly (IC 50 < 5 µg/mL) inhibited the growth of the chloroquino-resistant P. falciparum strain (Pf Dd2) with an IC 50 value of 2.78 ± 0.06 µg/mL, but was not active on the chloroquino-sensitive P. falciparum strain (Pf 3D7, IC 50 >100 µg/mL) (Table 2, Figure 4). AK-MeOH also exerted a selective activity against Pf Dd2 (SI > 179.86) with a non-deleterious effect on macrophages RAW 264.7 (IC 50 value > 500 µg/mL). Previous investigation of the methanolic stem and root bark extracts from A. klaineanum collected in the central region of Cameroon (Mount Eloumden) reported a very weak activity against P. falciparum 3D7 (IC 50 > 25 µg/mL) [30]. This discrepancy in activities as compared with the current result might be related to the plant's origin, which is also correlated to the secondary metabolites content [31]. In another study, dichloromethane extract from the stem bark of A. klaineanum displayed an antiplasmodial potential against the chloroquine-sensitive (3D7), with an IC 50 value of 16.7 µg/mL [9], thereby corroborating our findings to a certain extent. Except from the dichloromethane fraction (AK-DCM), all other fractions of AK-Hex and AK-EtOAc respectively displayed a strong antiplasmodial activity against the chloroquino-resistant P. falciparum strain (Pf Dd2) (IC 50 < 5 µg/mL) and a high selectivity toward Raw cells (SI > 94. 34 and SI > 100.40).  From these fractions (AK-Hex, AK-DCM, and AK-EtOAc), an unprecedented cerebroside (1) and known compounds (2-6) were isolated. Few studies report the phytochemical analysis of this plant species. However, plants belonging to the Anacardiaceae family were previously reported to contain phytosterols such as β-sitosterol, stigmasterol, and phenolic compounds like scopoletin (Pseudospondias macrocarpa) [32]. Regarding their activity, the new antroklaicerebroside (1) exhibited a moderate and selective antiplasmodial activity against Pf 3D7 with an IC 50 value of 42.04 ± 0.08 µg/mL and a selectivity index greater than 11. The phenolic compound 3,3 -dimethylellagic acid (4) and the coumarin-related compound scopoletin or 7-hydroxy-6-methoxycoumarin (3) were found to be non-active against both Pf 3D7 and Pf Dd2 (IC 50 > 50 µg/mL). Meanwhile, esculetin or 6,7-dihydroxycoumarin (2) displayed a moderate activity against Pf 3D7 and a promising activity against Pf Dd2 with IC 50 values of 45.57 and 9.3 µg/mL, respectively. Amongst the tested phytosterol compounds, only β-sitosterol exhibited a moderate antiplasmodial activity against the chloroquino-sensitive Pf 3D7 strain. Globally, with selectivity indexes greater than 10, all the test samples were found to be selective toward Raw 264.7 cells. Conversely, the methanol (AK-MeOH) root extract of Antrocaryon klaineanum showed no antileishmanial activity (IC 50 > 100 µg/mL). Further investigation of the later has resulted in more potent antileishmanial fractions, with increase in activity against L. donovani promastigotes. The ethyl acetate fraction (AK-EtOAc) exhibited the highest potency (IC 50 = 4.80 ± 0.13 µg/mL), followed by the hexane fraction (AK-Hex; 15.78 ± 0.82 µg/mL and a relatively high selectivity (SI > 104.17, SI > 31.69, respectively) ( Table 2). Compound (1) isolated from this plant selectively has showed moderate activity against L. donovani, with IC 50 of 6.19 ± 0.79 µg/mL. Except esculetin (2), which was not active (IC 50 > 50 µg/mL) on L. donovani, the other known compounds displayed good antileishmanial activity with IC 50 values ranging from 5.56 ± 0.74 to 9.14 ± 0.96 µg/mL, with β-sitosterol being the most active (IC 50 5.56 ± 0.74 µg/mL). The identified compounds mostly belong to metabolites reputed for their biological activities and with known antiparasitic modes of action. For instance, Mogana et al. [33] reported an approximately same range of activity for scopoletin isolated from the leaves of Canarium patentinervium (Burseraceae) with an IC 50 of 163.3 µg/mL against Leishmania donovani strain MHOM/IN/1983/AG83, an activity that was associated to its anticholinesterase activity (IC 50 51 µg/mL). In another study, this coumarin-related compound displayed the highest antileishmanial activity with an IC 50 value of 6.804 µg/mL [34]. The phenolic compound esculetin (2) is reputed for its antioxidant, anticoagulant, antibacterial, anti-inflammatory, and antidiabetic effects, according to Liang et al. [35]. On the other hand, β-sitosterol from Ifloga spicata (asteraceae) exhibited significant activity against leishmania promastigotes (IC 50 values of 9.2 ± 0.06 µg/mL) by targeting the DNA, inducing apoptosis, and through strong inhibition of two important leishmanial enzymes-namely, leishmanolysin and trypanothione reductase [36]. Later on, Corchorus capsularis L. leaf-derived β-sitosterol was found to exert its antileishmanial effects against Leishmania donovani by targeting trypanothione reductase and disrupting the parasite redox balance via intracellular ROS production in promastigotes [37]. 3,3 -dimethylellagic acid is a bioactive polyphenolic compound naturally present as a secondary metabolite in many plants. Indeed, a bioassay guided fractionation of Hymenostegia afzelii and Endodesmia calophylloides identified 3,3 -dimethylellagic as the highest antiplasmodial compound with an IC 50s of 4.27 µM and 1.36 µM against Pf 3D7 and Pf Dd2, respectively [38]. Moreover, ellagic acid exhibited an in vitro effect against Leishmania donovani. Interestingly, the same compounds significatively reduced parasitemia during an in vivo antileishmanial mouse model [39,40].     From these fractions (AK-Hex, AK-DCM, and AK-EtOAc), an unprecedented cerebroside (1) and known compounds (2-6) were isolated. Few studies report the phytochemical analysis of this plant species. However, plants belonging to the Anacardiaceae family were previously reported to contain phytosterols such as β-sitosterol, stigmasterol, and phenolic compounds like scopoletin (Pseudospondias macrocarpa) [32]. Regarding their activity, the new antroklaicerebroside (1) exhibited a moderate and selective antiplasmodial activity against Pf3D7 with an IC50 value of 42.04 ± 0.08 μg/mL and a selectivity index greater than 11. The phenolic compound 3,3′-dimethylellagic acid (4) and the coumarinrelated compound scopoletin or 7-hydroxy-6-methoxycoumarin (3) were found to be non-active against both Pf3D7 and PfDd2 (IC50 > 50 μg/mL). Meanwhile, esculetin or 6,7dihydroxycoumarin (2) displayed a moderate activity against Pf3D7 and a promising activity against PfDd2 with IC50 values of 45.57 and 9.3 μg/mL, respectively. Amongst the tested phytosterol compounds, only β-sitosterol exhibited a moderate antiplasmodial activity against the chloroquino-sensitive Pf3D7 strain. Globally, with selectivity indexes greater than 10, all the test samples were found to be selective toward Raw 264.7 cells. Conversely, the methanol (AK-MeOH) root extract of Antrocaryon klaineanum showed no antileishmanial activity (IC50 > 100 μg/mL). Further investigation of the later has resulted in more potent antileishmanial fractions, with increase in activity against L. donovani promastigotes. The ethyl acetate fraction (AK-EtOAc) exhibited the highest potency (IC50 = Finally, taken together, the current results are consistent with those reported in the literature, thereby confirming the traditional use of this plant.

Discussion
Using repeated chromatographic columns, six (1-6) compounds were isolated from the methanol crude extract of the root barks of Antrocaryon klaineanum Pierre (Anacardiaceae), including an unreported cerebroside (1) derivative, along with five known compounds: two coumarins (2-3), an ellargic acid (4), and two steroids (5)(6). The presence of coumarins, gallic acid, as well as steroids in the Antrocaryon genus or in the Antrocaryon klaineanum species has been previously reported [30,41]. In addition, coumarins and gallic acid have been reported to be a reliable chemophenotype of the Antrocaryon genus [7]. However, this is the first report of cerebroside (1) in the Antrocaryon genus as well as in the Antrocaryon klaineanum species. This phytochemical study is in agreement with the chemophenetic survey on Antrocaryon klaineanum. This study also improves the knowledge on the classes of secondary metabolite which can be found in this plant species.
The antiplasmodial activities of the methanol crude extract (AK-MeOH), n-hexane fraction (AK-Hex), ethyl acetate fraction (AK-EtOAc), dichloromethane fraction (AK-DCM), and compounds 1-6 were tested on the chloroquine-sensitive 3D7 and chloroquineresistant Dd2 strains of Plasmodium falciparum. The AK-MeOH (IC 50 , 2.78 ± 0.06 µg/mL), AK-EtOAc (IC 50 , 4.98 ± 0.81µg/mL), AK-Hex (IC 50 , 5.30 ± 0.19 µg/mL), and compound 2 (IC 50 , 9.30 ± 0.29 µg/mL) were strongly active against chloroquine-resistant Dd2 strain. The strong activity of the crude extract as well as the main fractions may be due to the synergic effect of compounds found in those extracts, and coumarins were the most active class of compounds. The above results clearly show that fractionation reduces the efficiency of the AK-MeOH. Moreover, AK-EtOAc, is the second-most active fraction, which can be due to the presence of coumarins and some hydrosoluble phenolic acids (ellargic acid) which are both classes of compounds that have shown an interesting antiplasmodial activity against Dd2 strain, as reported by Douanla et al. [9].
On the other hand, the AK-MeOH, AK-EtOAc, AK-Hex, and AK-DCM as well as compounds 1-6 were also evaluated for their antileishmanial activities against Leishmania donovani. The extract AK-MeOH, the fraction AK-DCM, and compound 2 reveal a low antileishmanial activity with an IC 50 > 50 µg/mL while the fraction AK-EtOAc as well as compounds 1,3-6 have shown a strong antileishmanial activity with an IC 50 ranging from 4.80 ± 0.13 to 9.14 ± 0.96 µg/mL (Table 2, Figure 4), with the fraction AK-EtOAc being the most active (IC 50 of 4.80 ± 0.13 µg/mL). Contrarily to the antiplasmodial activity, the fractionation increases the antileishmanial activity, meaning that compounds are antagonists in AK-MeOH, AK-Hex, and AK-DCM, while the AK-EtOAc activity could be due to the presence of active metabolites presenting a synergic effect. It is also noted that the compounds 1 (IC 50 , 6.19 ± 0.79 µg/mL), 3 (IC 50 , 6.40 ± 0.80 µg/mL), and 6 (IC 50 , 8.07 ± 0.90 and 9.14 ± 0.96 µg/mL, respectively) strongly inhibited the progmastigotes of Leishmania donovani responsible for viceral leishmaniasis. In addition, the most active compound [5 (IC 50 , 5.56 ± 0.74 µg/mL)] belongs to the class of coumarins, which is expected because coumarins act by damaging the mitochondrial membrane, causing ultrastructural changes in the leishmania parasite [42,43]. Indeed, the activity of compound 3 is stronger than that of its isomer (compound 2) due to the presence of the methoxy group present in compound 3 which can reduce the damage of the mitochondrial membrane.

General Experimental Procedures
The melting point was measured on an Electrothermal Engineering Ltd. serial N • 4386. The IR spectrum was recorded in KBr, on an Alpha spectrometer (Bruker, Billerica, MA, USA) spectrophotometer. Compounds were dissolved in 85 µL of their corresponding solvent in the NMR tube (Eurisotop ref. D009B) before being analyzed in the spectrometer type Bruker Avance II+ at 600 MHz equipped with a Cryoprobe TCI operating under

Plant Material
The root barks of Antrocaryon klaineanum Pierre (Anacardiaceae) were harvested by M. Olama Evouna Joseph Abraham in the Ossoebemva village of the Center Region of Cameroon on 10 July 2020. This plant had been identified in comparison with the specimen sample available at the Cameroon National Herbarium under the voucher 2124 SRF/CAM.

Extraction and Isolation
The air-dried root barks of A. klaineanum were used as plant material for this research work. Powdered plant material (4455.0 g) was extracted with methanol for 72 h in the percolator. The extract was evaporated to dryness using a rotary evaporator under reduced pressure leading to 628.4 g of crude extract. The methanol crude extract (600.0 g) was subjected to liquid-liquid extraction over hexane (10.4 g), dichloromethane (30.3 g), ethyl acetate (126.3 g), and residue. The EtOAc extract (120.0 g) was purified using repeated open column chromatography with silica gel as stationary phase with the system of n-hexane/dichloromethane and dichloromethane/methanol gradient, which led to the isolation of four compounds: 4 (9.3 mg), 3 (89.3 mg), 2 (19.2 mg), and 1 (3.1 mg). The n-hexane fraction and the dichloromethane fraction were both mixed together since their TLC profile was not so different. The purification of the combination led to the isolation of compounds 5 (201.3 mg) and 6 (901.4 mg).

Biological Activities 4.4.1. Parasite and Cell Culture
The cryopreserved chloroquine-sensitive (Pf 3D7-(MRA-102)), the multi-resistant (Pf Dd2) of Plasmodium falciparum, and the promastigote form of Leishmania donovani strains were obtained from BEI Resources (https://www.beiresources.org/ (accessed on 16 February 2019)) and are routinely cultured at the Antimicrobial and Biocontrol Agents Unit, University of Yaoundé I.
RAW 264.7 cells were obtained from RIKEN BioResource Centre Cell Bank (Tsukuba, Japan) and cultured in Dulbecco's modified Eagle medium (DMEM) containing 1% penicillinstreptomycin and 10% FBS under 5% CO 2 and humidified atmosphere at 37 • C and subcultured every 72 h.

Sample Preparation for Biological Assays
A stock solution was prepared at 100 mg/mL for each extract and fraction and at 50 mg/mL for isolated compound in 100% DMSO (Sigma-Aldrich, Munich, Germany). Master plates were then prepared by mixing 2 µL of each stock solution with 198 µL of fresh incomplete RPMI 1 640 culture medium to yield a concentration of 1 mg/mL for extract and fraction and of 0.5 mg/mL (1% DMSO) followed by a 5-fold serial dilution. Extracts and fractions were tested at concentrations ranging from 0.16 to 100 µg/mL, and isolated compounds at concentrations ranging from 0.08 to 50 µg/mL.

Antiplasmodial Activity of Extract, Fractions, and Compounds Synchronization of Parasite Culture
The use of mixed-stage cultures can enable the test molecules to interact with all the three stages (ring, trophozoite, and schizont) found during a 48-h life cycle of P. falciparum in culture. Moreover, starting the experiment with a synchronized ring stage culture provides the advantage of monitoring the growth inhibitory effect of test samples without a rise-up in parasitemia during the ring-trophozoite-schizont transitions. For this particular reason, parasites were synchronized at the ring stage by serial treatment with 5 % sorbitol for two days prior to each experiment with respect to Lambros and Vanderberg, 1979 [44] protocol.

SYBR Green I-Based Fluorescence Assay
Drug sensitivity assay was carried out in 96-well microtitration plates using the SYBR green I based fluorescence assay (Smilkstein et al., 2004) [45]. This assay is specifically based on the development of a strong fluorescence as a result of SYBR green entry and fixation to the exposed DNA in non-treated wells where cells have proliferated. The fact that human red blood host cells are anucleate allows the use of SYBR green for the proper monitoring of the growth of the malarial parasite.
The 96 wells flat-bottom assay plates with sorbitol-synchronized ring stage parasites (haematocrit: 1%, parasitaemia: 2%, 90 µL) were incubated in the presence of 10 µL of pre-diluted extracts, fractions, isolated compounds, and reference drugs [Chloroquine and Artemisinin (Sigma-Aldrich, Munich, Germany)] at different triplicate concentrations (100 µg/mL-0.16 µg/mL for extract/fractions and 50 µg/mL-0.08 µg/mL for compounds). After an incubation period of 72 h at 37 • C, 100 µL of the SYBR Green I solution prepared in a lysis buffer (0.2 µL of 10,000× SYBR Green I per mL of lysis buffer) consisting of Tris (20 mM; pH 7.5), EDTA (5 mM), saponin (0.008%; m/v), and Triton X-100 (0.08%; v/v) was added to each well, mixed twice gently with a multi-channel pipette, and incubated in the dark at 37 • C for 1 h. Fluorescence was measured using the microplate reader Infinite M200 (Tecan) at an excitation and emission wavelength of 485 and 538 nm, respectively. These data were normalized to percent control activity using Microsoft Excel software. The data analysis was performed with GraphPad Prism 8.0 software, fitting by nonlinear regression, and concentration-response curves were generated to determine the median inhibitory concentration (IC 50 ) for each sample.
The resistance index (RI) was determined as the ratio of the IC 50 of the samples on the multi-resistant strain Dd2 and the IC 50 on the chloroquine-sensitive strain 3D7 as reported by Douanla et al. [9]. RI values below 1 indicated inhibitors preferentially acting against the resistant strain and vice versa.

Antileishmanial Activity of Extract, Fractions, and Compounds
The antileishmanial activity of crude extracts, derived fractions, and isolated compounds against cultured L. donovani promastigotes was evaluated using the resazurin fluorimetric assay as described by Siqueira-Neto et al. (2010) [46]. Briefly, promastigotes from a logarithmic phase culture (4 × 10 5 cells/mL; 90 µL) were seeded in 96-well microplates and treated with 10 µL of inhibitors at different triplicate concentrations (100 µg/mL-0.16 µg/mL for extract/fractions and 50 µg/mL-0.08 µg/mL for compounds). Plates were incubated for 28 h at 28 • C, followed by the addition of 1 mg/mL resazurin (0.15 mg/mL in DPBS; Sigma, Darmstadt, Germany). The negative and positive controls were, respectively, 0.1% DMSO and amphotericin B (Sigma, Darmstadt, Germany) (10-0.016 µg/mL) treated wells. After an additional incubation for 44 h, plates were then read on a Magelan Infinite M200 fluorescence plate reader (Tecan, Männedorf, Switzerland) at excitation and emission wavelengths of 530 and 590 nm, respectively. For each sample, growth inhibition percentages were calculated with Microsoft Excel Software; then, from the nonlinear regression fit, concentration-response curves were generated to deduce the median inhibitory concentration (IC 50 ) using the GraphPad Prism 8.0 software.

In Vitro Cytotoxicity Assay
The murine macrophages, Raw 264.7 cells were grown in a T-25 cm 2 flask containing the complete Dulbecco's modified Eagle's medium (Sigma-Aldrich, Munich, Germany) supplemented with 10% FBS (Sigma-Aldrich, Munich, Germany), 1% non-essential amino acids, and 1% (v/v) penicillin-streptomycin (Sigma-Aldrich, Munich, Germany) and incubated at 37 • in a humidified atmosphere containing 5% CO 2 . For the experiments, 80-90% confluent cell culture was trypsinized, counted using a Neubauer haemacytometer, and seeded into a 96-well cell culture treated flat-bottomed plate (SARSTEDT, Inc. Newton, NC 28658, USA) at a cell density of 10 4 cells/well. Plates were incubated overnight to allow cell adhesion. Cells were then treated with serially diluted concentrations of crude extract, fractions, and isolated compounds for 48 h. Podophyllotoxin (Sigma-Aldrich, Munich, Germany) was used as positive control with the highest concentration of 10 µM. Afterwards, 10 µL of resazurin solution (0.15 mg/mL prepared in PBS) was added to each well, and the plates were additionally incubated for 4 h. Of important note, resazurin is a nonfluorescent, nontoxic, and cell-permeable blue dye which is used to quantify cell viability following its conversion into the highly fluorescent pink dye resorufin in response to changes in the reducing environment within the cytosol of the cell. The level of fluorescence that positively correlates with cell viability was measured using a Magellan Infinite M200 plate reader (Tecan, Germany) at an excitation wavelength of 530 nm and an emission wavelength of 590 nm. The growth proliferation percentage of cells was calculated with respect to the negative control. The concentration-response curves were plotted using inhibitory percentages versus the logarithm of the drug concentration to determine the concentration of drug that reduces cell viability by 50% (CC 50 ) using GraphPad Prism 8.0 software. Selectivity indices (SI) [SI = CC 50 /IC 50 ] were determined for each inhibitor based on their antiparasitic activity (IC 50 ) and cell cytotoxicity (CC 50 on Raw cells). Samples with SI greater than 10 were classified as poorly toxic toward Raw cells [47].

Conclusions
The chemical study of the stem bark of Antrocaryon klaineanum led to the isolation and characterized of six (1-6) secondary metabolites, among which an unreported glucocerebroside derivative antroklaicerebroside (1). The chemical result is in agreement with the chemotaxonomy of Antrocaryon klaineanum belonging to the Antrocaryon genus and to the Anacardiaceae family, while the biological results are in accordance with the previous literature as well as the traditional uses of this plant species in the treatment of hemorrhoid and malaria.